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A new type of capillary column for open‐tubular electrochromatography
Author(s) -
Zhao Yingxin,
Zhao Rui,
Shangguan Dihua,
Liu Guoquan
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200209)23:17<2990::aid-elps2990>3.0.co;2-7
Subject(s) - capillary electrochromatography , electrochromatography , monolithic hplc column , coating , chromatography , chemistry , silane , capillary action , silica gel , layer (electronics) , silylation , materials science , high performance liquid chromatography , capillary electrophoresis , catalysis , organic chemistry , composite material
A new type of open‐tubular C 18 ester‐bonded electrochromatographic column was prepared with sol‐gel technology, followed by an on‐column octadecyl silylation reaction. Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol‐gel precursor to form a thin coating layer on the wall of the fused‐silica capillary. The C 18 groups were introduced into the coating layer by on‐column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8×10 5 plates/m was achieved for a basic pentapeptide using the C 18 ester‐bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol‐gel‐coated capillaries, the C 18 ester‐bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage.