Premium
Comparison of different setups for one‐ and two‐dimensional capillary high‐performance liquid chromatography and pressurized capillary electrochromatography coupled on‐line with mass spectrometry
Author(s) -
Stahl Mark,
Jakob Andreas,
von Brocke Alexander,
Nicholson Graeme,
Bayer Ernst
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200209)23:17<2949::aid-elps2949>3.0.co;2-0
Subject(s) - capillary electrochromatography , chromatography , capillary action , mass spectrometry , chemistry , electrochromatography , high performance liquid chromatography , electrospray ionization , electrospray , capillary electrophoresis–mass spectrometry , analytical chemistry (journal) , capillary electrophoresis , materials science , composite material
A comparison of different separation methods (high‐performance liquid chromatography (HPLC), capillary HPLC (CHPLC) and pressurized capillary electrochromatography (pCEC)) coupled on‐line with mass spectrometry (MS) is undertaken using the separation of a crude extract of ergot fungus ( secalis cornuti) as an example. New and simple setups for a two‐dimensional CHPLC coupled on‐line with electrospray ionization (ESI)‐MS (2D‐CHPLC‐MS) as well as for capillary size‐exclusion chromatography performed under pCEC conditions and coupled on‐line with ESI‐MS (CSEC‐pCEC‐MS) are shown. In addition, an improved method for column packing is presented.