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Determination of glycoalkaloids and relative aglycones by nonaqueous capillary electrophoresis coupled with electrospray ionization‐ion trap mass spectrometry
Author(s) -
Bianco Giuliana,
SchmittKopplin Philippe,
Benedetto Giuseppe De,
Kettrup Antonius,
Cataldi Tommaso R. I.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200209)23:17<2904::aid-elps2904>3.0.co;2-1
Subject(s) - chemistry , chromatography , electrospray ionization , mass spectrometry , capillary electrophoresis , ion trap , electrospray , capillary electrophoresis–mass spectrometry , ammonium acetate , selected reaction monitoring , analytical chemistry (journal) , ion , acetic acid , tandem mass spectrometry , high performance liquid chromatography , organic chemistry
Glycoalkaloids are naturally occurring nitrogen‐containing compounds present in many species of the family Solanaceae , including cultivated and wild potatoes ( Solanum spp.), tomatoes ( Lycopersicon spp.), etc. These compounds have pharmacological and toxicological effects on humans due to their significant anticholinesterase activity and disruption of cell membranes. Herein is reported the development of a capillary electrophoresis (CE) method using nonaqueous (NA) separation solutions in combination with ion trap mass spectrometry (MS and MS/MS) detection for the identification and quantification of glycoalkaloids and their relative aglycones. A mixture 90:10 v/v of MeCN‐MeOH containing 50 m M ammonium acetate and 1.2 M acetic acid (applied voltage of 25.5 kV) was selected as a good compromise for the separation and detection of these compounds. The electrospray MS measurements were carried out in the positive ionization mode using a coaxial sheath liquid, methanol‐water (1:1) with 1% of acetic acid at a flow rate of 2.5 νL/min. Under optimized experimental conditions, the predominant ion was the protonated molecular ion ([M+H] + ) of solanidine ( m/z = 398), tomatidine ( m/z = 416), chaconine ( m/z = 852), solanine ( m/z = 868), and tomatine ( m/z = 1034). MS/MS experiments were carried out systematically by changing the relative collisional energy and monitoring the intensities of the fragment ions that were not high enough to allow better quantification than with the mother ions. The method was used for analyzing glycoalkaloids in potato extracts.