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A capillary electrophoresis and off‐line capillary electrophoresis/electrospray ionization‐quadrupole time of flight‐tandem mass spectrometry approach for ganglioside analysis
Author(s) -
Zamfir Alina,
Vukelić Željka,
PeterKatalinić Jasna
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200209)23:17<2894::aid-elps2894>3.0.co;2-q
Subject(s) - capillary electrophoresis , chemistry , chromatography , mass spectrometry , electrospray ionization , tandem mass spectrometry , electrospray , capillary electrophoresis–mass spectrometry , analytical chemistry (journal)
A systematic study for the optimization and implementation of high‐performance capillary electrophoresis (HPCE) in conjunction with negative ion electrospray ionization‐quadrupole time of flight‐tandem mass spectrometry (ESI‐QTOF‐MS/MS) for the analysis of complex glycolipids is described. The performance of the capillary electrophoresis (CE) and off‐line CE/ESI‐QTOF‐MS approach has been explored for screening a complex ganglioside mixture from bovine brain. All instrumental and solution parameters demonstrated to require special adjustment and to have the most substantial effect on the CE separation, abundance of product ions produced in a low‐energy collision‐induced dissociation (CID) process and their detection by MS/MS, when attempting to identify and sequence single ganglioside molecular species from CE eluted fractions. Upon optimization of the experimental parameters, an efficient methodology emerged providing the general basic requirements for combined CE/ESI‐MS analysis of this type of complex glycoconjugate.