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Evalution of capillary electrophoresis‐frontal analysis for the study of low molecular weight drug‐human serum albumin interactions
Author(s) -
Østergaard Jesper,
Schou Christian,
Larsen Claus,
Heegaard Niels H. H.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200209)23:17<2842::aid-elps2842>3.0.co;2-b
Subject(s) - capillary electrophoresis , chromatography , human serum albumin , electrophoresis , chemistry , albumin , affinity electrophoresis , lipophilicity , serum albumin , capillary action , drug , dextran , biochemistry , pharmacology , affinity chromatography , biology , enzyme , materials science , composite material
Capillary electrophoresis frontal analysis was applied to 12 low molecular weight compounds including 8 drug substances displaying a range of different properties with respect to binding affinity, binding location, structure, lipophilicity, charge at physiological pH, and electrophoretic mobility. It was found that capillary electrophoresis frontal analysis can be used as a general method to study and quantify drug‐human serum albumin interactions. The binding parameters obtained were consistent with literature values. Dextran was in some cases added to the run buffer to improve separation of the drug and human serum albumin plateau peaks. Results indicate that mobility differences between free and complexed human serum albumin give rise to only minor errors. Capillary electrophoresis frontal analysis was also found applicable to the study of human serum albumin drug displacement reactions. Low sensitivity of the UV‐detection system was found to be the major limitation of capillary electrophoresis frontal analysis. The method is simple, and minimal effort has to be put into method development, which makes it well suited for screening in early drug development.