Premium
A family of novel DNA sequencing instruments based on single‐photon detection
Author(s) -
Alaverdian Ludmila,
Alaverdian Sinok,
Bilenko Olga,
Bogdanov Iouri,
Filippova Elena,
Gavrilov Dmitry,
Gorbovitski Boris,
Gouzman Michael,
Gudkov Georgy,
Domratchev Sergey,
Kosobokova Olga,
Lifshitz Nadia,
Luryi Serge,
Ruskovoloshin Victor,
Stepoukhovitch Andrew,
Tcherevishnick Marina,
Tyshko Georgy,
Gorfinkel Vera
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:16<2804::aid-elps2804>3.0.co;2-9
Subject(s) - dna sequencing , fluorescence , computer science , dna , broadband , nanotechnology , computational biology , optoelectronics , physics , optics , materials science , biology , telecommunications , genetics
We have developed a family of high‐performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the “color‐blind” single‐photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.