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Velocity‐independent microfluidic flow cytometry
Author(s) -
Eyal Shulamit,
Quake Stephen R.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:16<2653::aid-elps2653>3.0.co;2-h
Subject(s) - microfluidics , flow cytometry , flow (mathematics) , cytometry , measure (data warehouse) , biological system , flow velocity , materials science , nanotechnology , mechanics , physics , computer science , data mining , biology , microbiology and biotechnology
Pressure‐driven flow in microfluidic channels is characterized by a distribution of velocities. This distribution makes it difficult to implement conventional flow cytometry data analysis. We have demonstrated a method to measure velocity as an independent parameter when performing microfluidic flow cytometry. This method allows velocity‐independent analysis of particles such as beads or cells, and allows flow cytometry analysis of extended objects, such as long DNA molecules. It allows accurate flow cytometry in transient and nonuniform flows. This general measurement method could be used in the future to measure the velocity of particles in a variety of existing microfluidic devices without the need for changes in their design.