Premium
DNA separation at a liquid‐solid interface
Author(s) -
Seo YoungSoo,
Samuilov Vladimir A.,
Sokolov Jonathan,
Rafailovich Miriam,
Tinland Bernard,
Kim Jaeseung,
Chu Benjamin
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:16<2618::aid-elps2618>3.0.co;2-w
Subject(s) - materials science , adsorption , persistence length , chemical physics , length scale , substrate (aquarium) , surface (topology) , thermal diffusivity , crystallography , chromatography , chemical engineering , chemistry , polymer , thermodynamics , geometry , physics , composite material , oceanography , mathematics , quantum mechanics , engineering , geology
We demonstrate that it is possible to separate a broad band of DNA on a solid substrate without topological obstacles. The mobility was found to scale with molecular size ( N ) as N –0.25 , while the resolution scaled as N 0.75 indicating that diffusivity on this substrate was minimal. By varying the buffer concentration we were able to show that the mobility for a given chain length scaled with the persistent length ( p ) as p 1/2 . This could be shown to be related to the Gaussian conformation of the chains adsorbed on the surface. A two‐dimensional corrugated surface of nonporous silica beads was produced using a self‐assembling process at the air/water interface. Even though the surface corrugations were comparable to persistence length we show that they do not affect the mobility, indicating that surface friction rather than topological constraints are the predominant mechanism of separation on a surface.