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Concentration gradient used in double‐stranded DNA separation by capillary electrophoresis
Author(s) -
Liang Dehai,
Chu Benjamin
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:16<2602::aid-elps2602>3.0.co;2-x
Subject(s) - capillary electrophoresis , capillary action , chromatography , range (aeronautics) , resolution (logic) , concentration gradient , electrophoresis , analytical chemistry (journal) , dna , chemistry , capillary length , density gradient , separation (statistics) , materials science , biochemistry , physics , quantum mechanics , artificial intelligence , machine learning , computer science , composite material
Effects of concentration gradient on double‐stranded DNA (dsDNA) separation by capillary electrophoresis are presented. By using a concentration gradient in the range between 0.8% and 3.2% for poly( N , N ‐dimethylacrylamide) (PDMA), the presence of a mesh‐size gradient in the capillary could enhance the separation of larger size DNA fragments, better than that based on a single uniform concentration over the same capillary length. A decrease in the column length could make the gradient effect more obvious. An optimal capillary length could be achieved by using a judicious combination of the concentration gradient and the concentration range, yielding a maximum resolution for the system. The standard deviation of the migration time measured for each DNA fragment was less than 5% in ten continuous runs, suggesting that the gradient formed inside the column was quite stable.