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Electrophoretic capture of circular DNA in gels
Author(s) -
Åkerman Björn,
Cole Kenneth D.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:16<2549::aid-elps2549>3.0.co;2-q
Subject(s) - electrophoresis , dna , electric field , microfluidics , plasmid , trapping , circular dna , polymer , trap (plumbing) , gel electrophoresis of nucleic acids , dna supercoil , materials science , chemistry , chromatography , biological system , nanotechnology , chemical physics , biophysics , biology , gene , physics , biochemistry , genome , dna replication , quantum mechanics , meteorology , composite material , ecology
Results on electrophoretic capture of circular DNA in porous gels are reviewed. Processes which cause arrest of circular forms of DNA during electrophoresis can provide very efficient separation mechanism for the purification of plasmids and bacterial artificial chromosomes if the corresponding linear form is not trapped and therefore removed by the electric field. Two types of such topological traps have been proposed, impalement and lobster traps, and we here review the present experimental support for the existence of these two circle‐specific mechanisms. Experiments designed to characterize the traps are discussed, regarding the concentration of the traps as well as their efficiency and capacity to trap both relaxed and supercoiled circular DNA. Studies of the dynamics of the capture process show that the average capture time is on the order of 10 s at 20 V/cm, by which time the circles have migrated several hundred micrometers and have passed hundreds of traps. We also review results on attempts to improve the capacity and efficiency of the trapping process by modification of the gels either by enzymatic treatment or by cogelation of neutral polymers.