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Performance of an ultraviolet light‐emitting diode‐induced fluorescence detector in capillary electrophoresis
Author(s) -
Hillebrand Sandro,
Schoffen Júlio Ricardo,
Mandaji Marcos,
Termigi Carlos,
Grieneisen Hans Peter Henrik,
Kist Tarso Benigno Ledur
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:15<2445::aid-elps2445>3.0.co;2-k
Subject(s) - capillary electrophoresis , fluorescamine , fluorescence , ultraviolet , chemistry , analytical chemistry (journal) , detector , detection limit , chromatography , diode , linear range , fluorescence spectroscopy , laser induced fluorescence , electrophoresis , materials science , optoelectronics , optics , physics
The performance of a fluorescence detector in capillary electrophoresis (CE) using a light‐emitting diode (LED) as excitation source is reported. An ultraviolet LED pulsed at a repetition rate of 500 Hz, combined with a time‐discrimination and averaging acquisition system, was used. Limits of detection of 3 and 18 fmoles (at a signal‐to‐noise ratio equal to 3) were achieved for fluorescamine‐derivatized bradykinin and lysine, respectively. This system exhibited a linear response for a concentration range between 54 and 417 ν M for derivatized lysine, and between 1.81 and 23.58 ν M for derivatized bradykinin. This detection system showed to be very convenient for routine analytical applications.