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Application of capillary electrophoresis with field‐amplified sample injection for the detection of new adrenoreceptor antagonist enantiomers in plasma in the low ng/mL concentration range
Author(s) -
Grard Sylvie,
Morin Philippe,
Ribet JeanPaul
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:15<2399::aid-elps2399>3.0.co;2-t
Subject(s) - capillary electrophoresis , chromatography , enantiomer , chemistry , analytical chemistry (journal) , capillary action , materials science , stereochemistry , composite material
Throughout the separation of chiral basic drugs by capillary electrophoresis (CE) with neutral hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) as chiral selector, the sensitivity of detection has been improved by using field‐amplified sample injection (FASI). In the present work, this on‐line stacking method has been used to detect low ng/mL levels of cationic enantiomers of a new adrenoreceptor antagonist in plasma. A systematic study of the parameters affecting on‐line concentration of these enantiomers (nature of the preinjection plug, composition of sample solvent, injection times of water and sample plugs) has been performed enabling the detection sensitivity of antagonist enantiomers to be improved by 180 times compared with usual hydrodynamic injection. The quantification of each adrenoreceptor antagonist enantiomer in plasma samples was then performed in the 2–100 ng/mL (or 8–400 n M ) concentration range after a solid‐phase extraction step. Using this FASI‐CE‐UV procedure, the limit of quantification (LOQ) for each enantiomer was in the low ng/mL concentration range (3 ng/mL or 10 n M ).