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An improved Western blotting technique effectively reduces background
Author(s) -
Wu Min,
Stockley Peter G.,
Martin William J.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200208)23:15<2373::aid-elps2373>3.0.co;2-w
Subject(s) - blot , buffer (optical fiber) , protocol (science) , chemistry , chromatography , computer science , biochemistry , telecommunications , medicine , alternative medicine , pathology , gene
We have developed a simple method to dramatically improve Western blotting by use of water washing as well as altered buffer conditions. Our improved protocol reduces the cost and labor of Western blotting by shortening the washing time and uses less buffer and other reagents. This procedure does not significantly change existing protocols of Western blotting analysis; it simply contains a few modifications to the washing step and buffer conditions. Our improved technique may therefore have broad applications in various experimental conditions.