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In‐capillary derivatization and analysis of amino acids, amino phosphonic acid‐herbicides and biogenic amines by capillary electrophoresis with laser‐induced fluorescence detection
Author(s) -
Molina Manuel,
Silva Manuel
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200207)23:14<2333::aid-elps2333>3.0.co;2-5
Subject(s) - capillary electrophoresis , derivatization , micellar electrokinetic chromatography , chemistry , chromatography , analyte , amino acid , laser induced fluorescence , resolution (logic) , detection limit , fluorescence , electrophoresis , capillary action , mass spectrometry , biochemistry , physics , quantum mechanics , artificial intelligence , computer science , materials science , composite material
This paper describes a general approach for the in‐capillary derivatization of amino compounds and the subsequent sensitive determination of the derivatives by micellar electrokinetic chromatography (MEKC) or capillary zone electrophoresis (CZE) with laser‐induced fluorescence (LIF) detection. Amino acids, biogenic amines and amino phosphonic acid‐herbicides were chosen as model analytes to evaluate the analytical potential of this approach. Fulfilment of the in‐capillary reaction of the analytes using LIF detection hinged on the excellent labeling chemistry of 5‐(4,6‐dichloro‐ s ‐triazin‐2‐ylamino)fluorescein (DTAF) and the good resolution achieved in the separation of derivatized analytes. Careful optimization of the electrophoretic conditions in the mixing step of this protocol allowed the determination of amino acids, biogenic amines and phosphorus‐containing amino acid‐herbicides with concentration limits of detection at the νg/L level and relative standard deviations from 3.5 to 5.8%. The whole analysis is carried out within 20 min, resulting in a very simple, fast and practical approach for the fully automated analysis of amino acids and related compounds in low‐volume and low‐concentration samples.