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The separation of oligodeoxynucleotides having a single‐base difference by affinity capillary electrophoresis using oligodeoxynucleotide‐ polyacrylamide conjugate
Author(s) -
Anada Takahisa,
Arisawa Takumi,
Ozaki Yoshihisa,
Takarada Tohru,
Katayama Yoshiki,
Maeda Mizuo
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200207)23:14<2267::aid-elps2267>3.0.co;2-i
Subject(s) - conjugate , polyacrylamide , capillary electrophoresis , chromatography , polyacrylamide gel electrophoresis , chemistry , electrophoresis , oligonucleotide , base (topology) , dna , biochemistry , polymer chemistry , mathematics , mathematical analysis , enzyme
We have developed an affinity capillary electrophoresis for gene mutation assay. We show a method for separating a mixture of oligodeoxynucleotides having single‐base difference using oligodeoxynucleotide‐polyacrylamide conjugate. Because the conjugate behaved similarly to nonionic polyacrylamide in terms of migration rate, it can be used as a pseudoimmobilized affinity ligand in a polyacrylamide‐coated capillary. Oligodeoxynucleotide having an oncogene sequence and a version with one base substituted were completely separated by this method. The magnesium‐ion concentration was found to be a key factor in achieving efficient separation of oligodeoxynucleotides with the same chain length.