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Determination of nitrite and nitrate in a proposed certified reference material for nutrients in seawater by capillary zone electrophoresis with artificial seawater as the background electrolyte using transient isotachophoresis
Author(s) -
Fukushi Keiichi,
Miyado Takashi,
Ishio Nobuhiro,
Nishio Hideaki,
Saito Keiitsu,
Takeda Sahori,
Wakida Shinichi
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200206)23:12<1928::aid-elps1928>3.0.co;2-k
Subject(s) - isotachophoresis , capillary electrophoresis , nitrite , seawater , chemistry , certified reference materials , nitrate , detection limit , electrolyte , chromatography , chloride , analytical chemistry (journal) , oceanography , organic chemistry , electrode , geology
We describe a combination of selected ions as a terminating ion which is useful for transient isotachophoresis (ITP) in capillary zone electrophoresis (CZE) for the determination of nitrite and nitrate in seawater. In addition to 150 m M sulfate as the principal terminating ion, 10 m M bromate was added to a sample solution as the additional terminating ion. Artificial seawater containing 3 m M cetyltrimethylammonium chloride (CTAC) was adopted as a background electrolyte (BGE). The limits of detection (LODs) for nitrite and nitrate were 2.2 and 1.0 νg/L (as nitrogen), respectively. The LODs were obtained at a signal to noise ratio (S/N) of 3. The values of the relative standard deviation (RSD) of peak area for these ions were 1.9 and 1.4%. The RSDs of peak height were 1.7 and 1.9%, the RSDs of migration time 0.11%. The proposed method was applied to the determination of nitrite and nitrate in a proposed certified reference material for nutrients in seawater, MOOS‐1, distributed by the National Research Council of Canada (NRC). The results almost agreed with the assigned tolerance interval.