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Capillary electrophoretic detection in apolipoprotein E genotyping
Author(s) -
Bekers Otto,
op den Buijsch Robert A. M.,
de Vries Johan E.,
Wijnen Petal A. H. M.,
van DieijenVisser Marja P.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200206)23:12<1878::aid-elps1878>3.0.co;2-g
Subject(s) - capillary electrophoresis , genotyping , apolipoprotein e , agarose gel electrophoresis , microbiology and biotechnology , polymerase chain reaction , agarose , electrophoresis , dna , chromatography , gel electrophoresis , genotype , biology , chemistry , gene , genetics , medicine , pathology , disease
We describe the application of capillary electrophoresis to detect DNA fragments, obtained after amplifying a part of the apolipoprotein E (apoE) gene with polymerase chain reaction (PCR). Compared to conventional agarose slab gel electrophoresis (AGE), CE appears the method of choice with regard to resolution and sensitivity, to detect DNA fragments in the range of 20–100 base pairs. Especially discrimination between apoE2/E2 and apoE2/E3 genotypes is more reliable with CE than with AGE, this being of great clinical value in the diagnosis of familiary dysbetalipoproteinemia.