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Characteristics of the electroosmotic flow of electrolyte systems for nonaqueous capillary electrophoresis
Author(s) -
Grob Miriam,
Steiner Frank
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200206)23:12<1853::aid-elps1853>3.0.co;2-d
Subject(s) - electrolyte , capillary electrophoresis , chemistry , acetonitrile , chromophore , analyte , electrophoresis , phosphoric acid , organic base , aqueous solution , electro osmosis , methanol , chromatography , inorganic chemistry , organic chemistry , electrode
Nonaqueous capillary electrophoresis (NACE) is a powerful tool for the analysis of surface‐active substances, which represent a broad class of analytes containing cationic and anionic species, such as surfactants, phosphoric acid esters, and amines. In order to conduct an efficient method development in NACE, the influence of the electrolyte composition on the electroosmotic flow (EOF) of organic separation systems was systematically investigated. Background electrolytes and background chromophores appropriate for direct and indirect UV‐detection were considered, as the majority of surface‐active substances do not absorb UV‐light. It was found that theoretical models developed to describe the EOF in aqueous electrolyte systems are insufficient for organic electrolyte systems. Experimental data on electroosmosis in a variety of organic solvents and mixtures of methanol and acetonitrile applying different background chromophores and basic or acidic additives are given. Differences between them are discussed with relation to the physicochemical properties of the organic solvents.