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The use of a highly sulfated cyclodextrin for the simultaneous chiral separation of amphetamine‐type stimulants by capillary electrophoresis
Author(s) -
Iwata Yuko T.,
Garcia Agnes,
Kanamori Tatsuyuki,
Inoue Hiroyuki,
Kishi Tohru,
Lurie Ira S.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200205)23:9<1328::aid-elps1328>3.0.co;2-z
Subject(s) - ephedrine , chemistry , capillary electrophoresis , chromatography , electrolyte , sulfation , cyclodextrin , pseudoephedrine , beta cyclodextrins , amphetamine , electrophoresis , phenylpropanolamine , phosphate buffered saline , biochemistry , pharmacology , medicine , electrode , neuroscience , dopamine , biology
We investigated the simultaneous chiral separation of nine amphetamine type stimulants ( dl ‐norephedrine, dl ‐norpseudoephedrine, dl ‐ephedrine, dl ‐pseudoephedrine, dl ‐amphetamine, dl ‐methamphetamine, dl ‐methylenedioxyamphetamine (MDA), dl ‐methylenedioxymethamphetamine (MDMA), and dl ‐methylenedioxyethylamphetamine (MDEA)) by capillary electrophoresis using highly sulfated γ‐cyclodextrin (SU(XIII)‐γ‐CD) as a chiral selector. Three different approaches using SU(XIII)‐γ‐CD with 50 m M phosphate background electrolyte were designed; (I) high CD concentration (10 m M SU(XIII)‐γ‐CD) at neutral pH (pH 7.0) in the normal polarity mode, (II) low CD concentration (1.0 m M ) at low pH (pH 2.6) in the normal polarity mode and (III) high CD concentration at low pH (pH 2.6) in the reversed‐polarity mode. In mode (II), the effects of adding three neutral CDs (β‐CD, dimethyl‐β‐CD and hydroxypropyl‐β‐CD) were also investigated. The best separation was obtained after optimizing mode (III) as follows: run buffer of 10 m M SU(XIII)‐γ‐CD with 50 m M phosphate background electrolyte at pH 2.6, applied voltage of –12 kV and capillary temperature of 15°C.