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Spherical molecularly imprinted polymer particles: A promising tool for molecular recognition in capillary electrokinetic separations
Author(s) -
de Boer Theo,
Mol Roelof,
de Zeeuw Rokus A.,
de Jong Gerhardus J.,
Sherrington David C.,
Cormack Peter A. G.,
Ensing Kees
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200205)23:9<1296::aid-elps1296>3.0.co;2-2
Subject(s) - molecularly imprinted polymer , capillary electrophoresis , methacrylic acid , chemistry , chromatography , electrokinetic phenomena , acetonitrile , precipitation polymerization , polymerization , polymer , aqueous solution , capillary action , methacrylate , colloid , electrophoresis , solvent , molecular imprinting , radical polymerization , materials science , selectivity , organic chemistry , composite material , catalysis
Spherical molecularly imprinted polymer particles obtained via precipitation polymerization, were introduced as a pseudostationary phase in capillary electrophoresis (CE) to study molecular recognition. Analyses were performed via a partial filling technique using (+)‐ephedrine‐imprinted microspheres (100–200 nm) which were polymerized from methacrylic acid and 1,1,1‐Tris(hydroxymethyl)propanetrimethacrylate using acetonitrile as the solvent. The influence of pH and the modifier content on the separation was investigated. A 0.1% w/v suspension in an aqueous 10 m M phosphate buffer (pH 2.5 with 40% acetonitrile) was hydrodynamically injected into the CE system (80% of the effective capillary length) and led to full baseline separation of racemic ephedrine within 10 min.