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A nanoliter rotary device for polymerase chain reaction
Author(s) -
Liu Jian,
Enzelberger Markus,
Quake Stephen
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200205)23:10<1531::aid-elps1531>3.0.co;2-d
Subject(s) - microfluidics , polymerase chain reaction , microfluidic chip , sample (material) , lab on a chip , chip , nanotechnology , power consumption , temperature cycling , sample preparation , process engineering , materials science , biochemical engineering , chromatography , computer science , chemistry , power (physics) , thermal , engineering , gene , biochemistry , telecommunications , physics , quantum mechanics , meteorology
Polymerase chain reaction (PCR) has revolutionized a variety of assays in biotechnology. The ability to implement PCR in disposable and reliable microfluidic chips will facilitate its use in applications such as rapid medical diagnostics, food control testing, and biological weapons detection. We fabricated a microfluidic chip with integrated heaters and plumbing in which various forms of PCR have been successfully demonstrated. The device uses only 12 nL of sample, one of the smallest sample volumes demonstrated to date. Minimizing the sample volume allows low power consumption, reduced reagent costs, and ultimately more rapid thermal cycling.