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Comb‐like copolymers as self‐coating, low‐viscosity and high‐resolution matrices for DNA sequencing
Author(s) -
Barbier Valessa,
Buchholz Brett A.,
Barron Annelise E.,
Viovy JeanLouis
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200205)23:10<1441::aid-elps1441>3.0.co;2-h
Subject(s) - polyacrylamide , copolymer , coating , grafting , polymer , viscosity , materials science , capillary action , chemical engineering , intrinsic viscosity , polymer chemistry , nanotechnology , composite material , engineering
Comb‐like copolymers with a polyacrylamide backbone and poly( N , N ‐dimethylacrylamide) grafts were prepared, as a way to combine the superior sieving properties of polyacrylamide with the self‐coating properties of polydimethylacrylamide. These matrices function well in the absence of a capillary coating, and achieve separation performances for single‐stranded DNA that are comparable to those of state‐of‐the‐art long‐chain linear polyacrylamide. Structural parameters such as the grafting density and the polymer molecular mass were varied, and good performance appears to be achieved with a relatively large range of parameters. Surprisingly, excellent separation is achieved even with matrices that have a viscosity as low as 200 mPa/s. A discussion of the physics underlying this behavior is provided.