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Pyrenebutanoate as a dynamic protein modifier for fluorometric detection in capillary zone electrophoresis
Author(s) -
Horká Marie,
Šlais Karel
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200204)23:7/8<1090::aid-elps1090>3.0.co;2-d
Subject(s) - capillary electrophoresis , chromatography , chemistry , capillary action , fluorescence , electrophoresis , analytical chemistry (journal) , materials science , physics , quantum mechanics , composite material
Pyrenebutanoate as the amphiphilic fluorescent compound is suggested as a buffer additive in capillary zone electrophoresis (CZE) for a dynamic modification of several protein samples. Using deuterium lamp UV excitation for the on‐column fluorometric detection, minimum detectable amounts in the amol‐range of the proteins sampled on the CZE capillary was achieved.