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Chiral separation of gemifloxacin in sodium‐ containing media using chiral crown ether as a chiral selector by capillary and microchip electrophoresis
Author(s) -
Cho Seung II,
Lee KookNyung,
Kim YongKweon,
Jang Jaeho,
Chung Doo Soo
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200203)23:6<972::aid-elps972>3.0.co;2-f
Subject(s) - capillary electrophoresis , chemistry , enantiomer , crown ether , chromatography , ether , amine gas treating , sodium , electrophoresis , ion , organic chemistry
Chiral crown ether, (+)‐(18‐crown‐6)‐tetracarboxylic acid (18C6H 4 ), is an effective chiral selector for resolving enantiomeric primary amines owing to the difference in affinities between 18C6H 4 and each of the amine enantiomers. In addition to the destacking effect of sodium ion in the sample solution, the strong affinity of sodium ion to the polyether ring of crown ether is unfavorable to chiral capillary electrophoresis using 18C6H 4 as a chiral selector. In this report, the chiral separation of gemifloxacin dissolved in a saline sample matrix using 18C6H 4 was investigated. Adding a chelating agent, ethylenediaminetetraacetic acid (EDTA), to the run buffer greatly improved the separation efficiencies and peak shapes. The successful chiral separation of gemifloxacin in a urinary solution was demonstrated for both capillary and microchip electrophoresis.