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Drug‐plasma protein binding assay by electrokinetic chromatography‐frontal analysis
Author(s) -
Ishihama Yasushi,
Miwa Toshinobu,
Asakawa Naoki
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200203)23:6<951::aid-elps951>3.0.co;2-r
Subject(s) - chemistry , chromatography , capillary electrophoresis , blood proteins , electrokinetic phenomena , ultrafiltration (renal) , electrophoresis , plasma , plasma protein binding , biochemistry , physics , quantum mechanics
We developed a rapid, microscale and reliable analytical method for binding of drugs to plasma proteins using capillary electrophoresis (CE) with ionic cyclodextrins (CD) combined with frontal analysis. These CDs were used as pseudostationary phases of electrokinetic chromatography (EKC). The CD‐modified EKC (CDEKC) approach allowed us to separate anionic drugs from plasma proteins, whereas CZE could not separate these drugs from plasma proteins because they had a similar mobility like plasma proteins. CDs uniquely interact with these drugs but not with plasma proteins. Therefore, CDEKC could be coupled with frontal analysis to measure the binding of anionic drugs to plasma proteins. The binding values obtained by CDEKC were highly consistent with those determined by the ultrafiltration method. Our CDEKC approach should expand the applicability of CE to protein binding analysis.