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Brownian motion of single molecules in electric fields
Author(s) -
Rigler Rudolf
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200203)23:5<805::aid-elps805>3.0.co;2-#
Subject(s) - electric field , chemistry , molecule , brownian motion , dipole , fluorescence , rhodamine , ion , atomic physics , molecular physics , analytical chemistry (journal) , excited state , chemical physics , biophysics , physics , optics , chromatography , quantum mechanics , organic chemistry , biology
The behavior of negatively charged single rhodamine‐labeled molecules excited to fluorescence in confocal volume elements has been investigated in oscillating voltage gradients between 1 and 15 kV/cm. The effective charge of rhodamin labeled deoxy‐uridine triphosphate (Rh‐dUTP) is dependent on the field strength applied and likely is due to differences in the screening by counter ions. It can be shown that the attractive forces generated by an oscillating dipole can increase the concentration of Rh‐dUTP by a factor 2. Likewise a substantial increase of concentration can be obtained for Rh‐labeled DNA molecules when linear voltage gradients are applied. The importance for diagnostic analysis at the single molecule level is discussed.