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Generation of minimal protein identifiers of proteins from two‐dimensional gels and recombinant proteins
Author(s) -
Schmidt Frank,
Lueking Angelika,
Nordhoff Eckhard,
Gobom Johan,
Klose Joachim,
Seitz Harald,
Egelhofer Volker,
Eickhoff Holger,
Lehrach Hans,
Cahill Dolores J.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:4<621::aid-elps621>3.0.co;2-j
Subject(s) - recombinant dna , mass spectrometry , identifier , matrix assisted laser desorption/ionization , chemistry , computational biology , chromatography , biochemistry , computer science , biology , desorption , gene , organic chemistry , adsorption , programming language
We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)‐mass spectrometry, termed a “minimal protein Identifier” (MPI). MPIs can be determined for proteins from two‐dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.