Premium
Towards disposable lab‐on‐a‐chip: Poly(methylmethacrylate) microchip electrophoresis device with electrochemical detection
Author(s) -
Wang Joseph,
Pumera Martin,
Chatrathi Madhu Prakash,
Escarpa Alberto,
Konrad Renate,
Griebel Anja,
Dörner Wolfgang,
Löwe Holger
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:4<596::aid-elps596>3.0.co;2-c
Subject(s) - capillary electrophoresis , amperometry , materials science , detector , chromatography , electrochemistry , electrophoresis , capillary action , relative standard deviation , chip , microfluidics , analytical chemistry (journal) , nanotechnology , detection limit , electrode , chemistry , computer science , composite material , telecommunications
A fully disposable microanalytical device based on combination of poly(methylmethacrylate) (PMMA) capillary electrophoresis microchips and thick‐film electrochemical detector strips is described. Variables influencing the separation efficiency and amperometric response, including separation voltage or detection potential are assessed and optimized. The versatility, simplicity and low‐cost advantages of the new design are coupled to an attractive analytical performance, with good precision (relative standard deviation RSD = 1.68% for n = 10). Applicability for assays of mixtures of hydrazine, phenolic compounds, and catecholamines is demonstrated. Such coupling of low‐cost PMMA‐based microchips with thick‐film electrochemical detectors holds great promise for mass production of single‐use micrototal analytical systems.