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Separation of DNA fragments in hydroxylated poly(dimethylacrylamide) copolymers
Author(s) -
Chiari Marcella,
Cretich Marina,
Consonni Roberto
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:4<536::aid-elps536>3.0.co;2-#
Subject(s) - copolymer , dna , chemistry , polymer science , polymer chemistry , materials science , polymer , organic chemistry , biochemistry
The novel polymer matrices reported here are low‐viscosity sieving media for DNA capillary electrophoresis. This new family of matrices comprises copolymers of N , N ‐dimethylacrylamide with different monomers which increase polymer hydrophilicity. All these new copolymers self‐coat on fused‐silica capillaries. Resolution, peak spacing and peak width were the parameters taken into account to assess the influence of polymer structure on separation selectivity and efficiency. This work demonstrates that the performance of polydimethylacrylamide (PDMA) can be improved through copolymerization with hydrophilic monomers. The improvement is related to the efficiency parameter. The new copolymers, due to their low viscosity high sieving capacity and ability to suppress EOF, represent a better alternative to PDMA and are suitable replaceable matrices for capillary and microchip electrophoresis.