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Using nonaqueous capillary electrophoresis to analyze several quinolones in pig kidney samples
Author(s) -
Hernández Margarita,
Borrull Francesc,
Calull Marta
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:3<506::aid-elps506>3.0.co;2-c
Subject(s) - danofloxacin , chemistry , chromatography , capillary electrophoresis , ammonium acetate , acetic acid , extraction (chemistry) , oxolinic acid , ammonium oxalate , hydrochloric acid , electrolyte , enrofloxacin , high performance liquid chromatography , ciprofloxacin , biochemistry , nalidixic acid , inorganic chemistry , electrode , antibiotics
We show the potential of nonaqueous capillary electrophoresis (NACE) for analyzing enrofloxacin (ENR), ciprofloxacin (CPR), danofloxacin (DAN), difloxacin (DIF), marbofloxacin (MAR), flumequine (FLU), and oxolinic acid (OXA) in pig kidney samples. We have studied the effects of parameters such as the composition of the organic media, the choice of electrolyte, the pH* of the background electrolyte (BGE), the addition of modifiers, and the reversal of electroosmotic flow. Separation was good with 20 m M ammonium acetate, 0.004% polycation hexadimethrine bromide (HDB), and 4% acetic acid (pH* 5.4) in methanol/acetonitrile (50:50 v/v) medium. We used a quick and simple sample preparation method, hydrochloric acid as an extractant and solid‐phase extraction (SPE) with Baker C 18 cartridges as the cleanup step. Recoveries for all quinolones were over 80%.