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Enantioseparations in nonaqueous capillary liquid chromatography and capillary electrochromatography using cellulose tris(3,5‐dimethylphenylcarbamate) as chiral stationary phase
Author(s) -
Chankvetadze Lali,
Kartozia Irma,
Yamamoto Chiyo,
Chankvetadze Bezhan,
Blaschke Gottfried,
Okamoto Yoshio
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:3<486::aid-elps486>3.0.co;2-l
Subject(s) - capillary electrochromatography , chromatography , chemistry , high performance liquid chromatography , cellulose , capillary action , capillary electrophoresis , phase (matter) , silica gel , supercritical fluid chromatography , organic chemistry , materials science , composite material
The potential of the widely used chiral stationary phase for high‐performance liquid chromatography (HPLC) enantioseparations, cellulose tris(3,5‐dimethylphenylcarbamate) (CDMPC, sold under the trade name Chiralcel OD) was evaluated under the conditions of nonaqueous capillary electrochromatography (CEC). The effect of the particle size of the silica gel, the loading of CDMPC on the silica gel and nature of the organic solvent, as well as electrolyte salts on the separation characteristics were investigated. This study illustrates the applicability of CDMPC for obtaining highly efficient enantioseparations under the conditions of nonaqueous CEC. Comparative study of enantioseparations in capillary liquid chromatography (CLC) and CEC indicated the significant advantages of CEC such as higher plate number at the similar linear flow rates of the mobile phase as well as better tolerance of higher linear flow rates.