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Use of vancomycin silica stationary phase in packed capillary electrochromatography: III. Enantiomeric separation of basic compounds with the polar organic mobile phase
Author(s) -
Fanali Salvatore,
Catarcini Paolo,
Quaglia Maria Giovanna
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:3<477::aid-elps477>3.0.co;2-l
Subject(s) - capillary electrochromatography , enantiomer , chemistry , ammonium acetate , chromatography , acetonitrile , phase (matter) , solvent , silica gel , electrochromatography , packed bed , methanol , high performance liquid chromatography , capillary electrophoresis , organic chemistry
The separation of basic compounds into their enantiomers was achieved using capillary electrochromatography in 50 or 75 νm inner diameter (ID) fused‐silica capillaries packed with silica a stationary phase derivatized with vancomycin and mobile phases composed of mixtures of polar organic solvents containing 13 m M ammonium acetate. Enantiomer resolution, electroosmotic flow, and the number of theoretical plates were strongly influenced by the type and concentration of the organic solvent. Mobile phases composed of 13 m M ammonium acetate dissolved in mixtures of acetonitrile/methanol, ethanol, n ‐propanol, or isopropanol were tested and the highest enantioresolutions were achieved using the first mobile phase, allowing the separation of almost all investigated enantiomers (9 from 11 basic compounds). The use of capillaries with different ID (50 and 75 νm ID) packed with the same chiral stationary phase revealed that a higher number of theoretical plates and higher enantioresolution was achieved with the tube with lowest ID.*