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Nonaqueous affinity capillary electrophoresis investigation of small molecule molecular recognition
Author(s) -
Peddicord Michael B.,
Weber Stephen G.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:3<431::aid-elps431>3.0.co;2-o
Subject(s) - morpholine , chemistry , acetonitrile , methanol , capillary electrophoresis , hydrogen bond , solvent , protonation , acetic acid , molecule , chromatography , medicinal chemistry , organic chemistry , ion
The conditional binding constants for a bis ‐guanidinium‐like receptor and a series of dicarboxylate ligands have been determined in two buffer/solvent systems, namely 25 m M ammonium acetate/1% acetic acid in acetonitrile/methanol (7:3 v:v) and 30 m M N ‐methyl morpholine/15 m M methanesulfonic acid in acetonitrile/methanol (9:1 v:v). The latter buffer has not been applied before in capillary electrophoresis. The binding constants in both solvent systems decrease as the dicarboxylate length increases. The binding constants are larger in the less competitive N ‐methyl morpholine buffer. The dicarboxylates associate only weakly with a dicationic analog of the receptor, p ‐xylyl trimethylammonium, which is not a hydrogen bond donor.