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Comparison of aqueous and nonaqueous carrier electrolytes for the separation of penicillin V and related substances by capillary electrophoresis with UV and mass spectrometric detection
Author(s) -
Hilder Emily F.,
Klampfl Christian W.,
Buchberger Wolfgang,
Haddad Paul R.
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:3<414::aid-elps414>3.0.co;2-k
Subject(s) - chemistry , capillary electrophoresis , chromatography , electrolyte , aqueous solution , acetonitrile , solvent , methanol , analyte , detection limit , electrospray , isotachophoresis , selectivity , mass spectrometry , organic chemistry , electrode , catalysis
A method for the determination of penicillin V together with its impurities and by‐products formed during biosynthesis, using capillary electrophoresis (CE) with UV and electrospray‐mass spectrometric (ESI‐MS) detection is presented. Aqueous and nonaqueous electrolytes containing 20 m M ammonium acetate were investigated to determine their suitability for the separation of these analytes. These carrier electrolytes were optimized with respect to the pH and the solvent/s used (water, methanol, acetonitrile, ethanol and isopropanol) and it was shown that although the nonaqueous electrolytes offered unique separation selectivities, the best results in terms of selectivity and sensitivity were obtained for the aqueous system. Finally, the applicability of this method for the analysis of a mixture representative of a real fermentation broth was demonstrated using an aqueous carrier electrolyte with both UV and ESI‐MS detection.