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Electrophoretic behavior of adamantane derivatives possessing antiviral activity and their determination by capillary zone electrophoresis with indirect detection
Author(s) -
Reichová Nad’a,
Pazourek Jiří,
Polášková Pavla,
Havel Josef
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:2<259::aid-elps259>3.0.co;2-u
Subject(s) - adamantane , rimantadine , capillary electrophoresis , chemistry , detection limit , chromatography , methylamine , electrophoresis , electrolyte , amine gas treating , amantadine , organic chemistry , virus , electrode , virology , neuroscience , influenza a virus , biology
Separation and determination of adamantane derivatives with antiviral activity, namely amantadine (1‐adamantan amine), memantine (1‐amino‐3,5‐dimethyl adamantane) and rimantadine (α‐methyl‐1‐adamantane methylamine), were examined by capillary zone electrophoresis. After optimization, an indirect detection method using 5 m M 4‐methylbenzylamine in ethanol/water solution (1:4) as simultaneously absorbing and buffering background electrolyte with detection at 210 nm was found suitable for determination of the individual compounds (limit of detection was 0.35 mgL –1 for memantine hydrochloride, S/N = 3). Baseline separation of all the three compounds was reached by addition of α‐ or β‐cyclodextrins to the electrolyte in concentrations of 20 and 2 m M , respectively.