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Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography
Author(s) -
Štěrbová Hana,
Ševčíková Petra,
Kvasničková Leona,
Glatz Zdeněk,
Slanina Jiří
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:2<253::aid-elps253>3.0.co;2-h
Subject(s) - micellar electrokinetic chromatography , chromatography , capillary electrochromatography , chemistry , capillary electrophoresis , electrolyte , sodium dodecyl sulfate , acetonitrile , electrokinetic phenomena , capillary action , high performance liquid chromatography , electrochromatography , reproducibility , analytical chemistry (journal) , materials science , electrode , composite material
Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimized with respect to the different parameters including sodium dodecyl sulfate (SDS) and acetonitrile concentration, pH of the background electrolyte, separation voltage, and capillary temperature. The background electrolyte consisting of 40 m M SDS and 35% acetonitrile in 10 m M tetraborate buffer (pH 9.3) was found to be the most suitable electrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the capillary temperature 25°C gave the best separation of lignans. The interday reproducibility of the peak areas and the migration times was below 2.0%. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed‐phase high‐performance liquid chromatography (RP‐HPLC). The possibilities of using this method for the determination of lignans in drug and in serum samples were also tested.