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Repartition effect of aromatic polyaniline coatings on the separation of bioactive peptides in capillary electrophoresis
Author(s) -
Bossi Alessandra,
Piletsky Sergey A.,
Turner Anthony P. F.,
Righetti Pier Giorgio
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:2<203::aid-elps203>3.0.co;2-0
Subject(s) - capillary electrophoresis , polyaniline , derivatization , capillary action , chromatography , coating , capillary electrochromatography , chemistry , electrophoresis , polymer , analyte , amine gas treating , materials science , mass spectrometry , organic chemistry , polymerization , composite material
The capillary walls of fused‐silica capillary electrophoresis (CE) columns were modified with a thin film of polyaniline (PANI), providing open‐tubular columns with a stable coating containing aromatic groups and amine functionalities. Fast and efficient separations were observed for small bioactive peptides under acidic conditions on PANI‐coated columns. The mechanism of separation is based on hydrophobic interactions between the analytes and the polymeric matrix. Good reproducibility was observed from run‐to‐run. Due to the simple derivatization procedure, method flexibility, the uniformity of the coating and its stability, conjugated polymers could find practical application in capillary zone electrophoresis (CZE) separations.