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Lipoproteins modulate growth and differentiation of cultured human epidermal keratinocytes
Author(s) -
Furumoto Hiroko,
Arai Hirofumi,
Kuramitsu Yasuhiro,
Saeki Yuri,
Gondo Toshikazu,
Ishihara Tokuhiro,
Shimizu Takahiro,
Fujimoto Masanori,
Nakamura Kazuyuki
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200202)23:2<161::aid-elps161>3.0.co;2-p
Subject(s) - very low density lipoprotein , ldl receptor , lipoprotein , receptor , chemistry , cell growth , keratinocyte , low density lipoprotein , apolipoprotein e , microbiology and biotechnology , biochemistry , cholesterol , biology , medicine , in vitro , disease
The effects of various lipoproteins on the growth and the differentiation of cultured normal human keratinocytes were invertigated. Primary cultures of human epidermal keratinocytes were obtained from neonatal foreskin, and then added with lipoproteins, very low density lipoprotein (VLDL), low density lipoprotein (LDL), and high density lipoprotein (HDL). Cell growth potential was examined using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay. VLDL and LDL enhanced keratinocytes growth and LDL receptor expression at the plasma membrane level. These effects were more remarkably observed in cells cultured with VLDL than in cells cultured with LDL. Apolipoprotein E (ApoE) was highly increased in VLDL treated cells. These results suggest that VLDL binds with high affinity to cell surface receptors and induces cell proliferation.