Premium
Maize pollen enzymes after two‐dimensional polyacrylamide gel electrophoresis in the presence or absence of sodium dodecyl sulfate
Author(s) -
Kalinowski Andrzej,
Radłowski Marek,
Bartkowiak Sławomir
Publication year - 2002
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200201)23:1<138::aid-elps138>3.0.co;2-d
Subject(s) - isoelectric focusing , polyacrylamide gel electrophoresis , gel electrophoresis , biochemistry , sodium dodecyl sulfate , chemistry , malate dehydrogenase , alcohol dehydrogenase , chromatography , dehydrogenase , enzyme
Twelve enzymes from mature pollen grains of maize were separated by two‐dimensional polyacrylamide gel electrophoresis (2‐D PAGE). The separation in the second dimension was both in the presence and absence of sodium dodecyl sulfate (SDS). Ten of the investigated enzymes lost activity after separation in the presence of SDS, but those of esterases and acid phosphatase could be recovered. On the other hand, 2‐D electrophoresis without SDS is suitable for the analysis of maize pollen pectinesterase, malate dehydrogenase, glutamic‐oxalacetic transaminase, diaphorase, superoxide dismutase, and phosphoglucose isomerase. 1‐D PAGE and isoelectric focusing (IEF) are sufficient to analyze glucose‐6‐phosphate dehydrogenase, alcohol dehydrogenase, shikimic dehydrogenase, and glutamate dehydrogenase. The possibility of applying 2‐D electrophoresis for the analysis of enzymes from single stigma and stigma exudate is dicussed.