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High‐throughput polymerase chain reaction analysis of clinical samples by capillary electrophoresis with UV detection
Author(s) -
Zhang Yonghua,
He Yan,
Yeung Edward S.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20017)22:11<2296::aid-elps2296>3.0.co;2-h
Subject(s) - capillary electrophoresis , polymerase chain reaction , chromatography , polymerase chain reaction optimization , dna , chemistry , electrophoresis , microbiology and biotechnology , capillary action , str analysis , throughput , biology , multiplex polymerase chain reaction , microsatellite , biochemistry , gene , materials science , allele , computer science , telecommunications , wireless , composite material
Routine genetic analysis of large numbers of individuals by polymerase chain reaction (PCR) using capillary electrophoresis is often restricted by the low throughput of standard protocols and the tedious sample preparation process. Here, we demonstrate that capillary electrophoresis with UV detection can be used in PCR‐based DNA analysis starting from clinical samples without purification or complicated sample manipulation. After PCR reaction using cheek cells, blood, or HIV‐1 gag DNA, the reaction mixtures were injected into a capillary array either on‐line or off‐line by base stacking. The use of multiplexed absorption detection and the elimination of any purification steps both before and after PCR reaction can potentially provide significant benefits compared to current methods for DNA analysis with regard to time, cost, and labor.