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Microchip isoelectric focusing using a miniature scanning detection system
Author(s) -
Raisi Fariba,
Belgrader Phillip,
Borkholder David A.,
Herr Amy E.,
Kintz Gregory J.,
Pourhamadi Farzad,
Taylor Michael T.,
Northrup M. Allen
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20017)22:11<2291::aid-elps2291>3.0.co;2-a
Subject(s) - detector , photomultiplier , isoelectric focusing , microchannel plate detector , microchannel , materials science , tube (container) , resolution (logic) , optics , chromatography , analytical chemistry (journal) , chemistry , nanotechnology , physics , computer science , artificial intelligence , biochemistry , composite material , enzyme
A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the real‐time distribution of fluorescently labeled peptides subjected to gel‐free mIEF. During the run, the effective length of the 6‐cm channel was scanned every 9 s. Analysis was completed within 5 min while still obtaining high resolution and sensitivity. In addition, the scanning detector was used to characterize peptide migration properties within the channel by providing simultaneous temporal and spatial measurements.