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Capillary electrophoresis of high‐molecular chitosan: The natural carbohydrate biopolymer
Author(s) -
Ban Eunmi,
Choi OneKyun,
Ryu JaeChun,
Yoo Young S.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20017)22:11<2217::aid-elps2217>3.0.co;2-r
Subject(s) - chitosan , capillary electrophoresis , biopolymer , chromatography , chemistry , capillary action , electrophoresis , phosphate buffered saline , correlation coefficient , analytical chemistry (journal) , polymer , materials science , organic chemistry , statistics , mathematics , composite material
Abstract Due to its high resolving power and diverse application range, capillary electrophoresis (CE) has been successfully applied to the analysis of carbohydrates. In this paper, a method for the determination of high‐molecular chitosan ( M r 200 000) using CE is presented. We studied the optimal condition of buffer pH and type, and column type for determination of chitosan. Optimal CE performance was found when employing 100 m M triethylamine (TEA)‐phosphate buffer, pH 2.0 and untreated fused‐silica capillary (50 νm×27 cm) for the chitosan analysis. Under optimum conditions, excellent linear responses were obtained in the concentration range of 1.25–20 ν M , with a linear correlation coefficient of 0.9983. The standard deviations of the migration time and peak area were found to be 2.5 and 6.4%, respectively. This method could be readily applied to chitosan determination in real biological samples and commercial products.