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A proteome database of human primary T helper cells
Author(s) -
Nyman Tuula A.,
Rosengren Arsi,
Syyrakki Saija,
Pellinen TimoPekka,
Rautajoki Kirsi,
Lahesmaa Riitta
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200112)22:20<4375::aid-elps4375>3.0.co;2-p
Subject(s) - proteome , proteomics , peptide mass fingerprinting , database , gel electrophoresis , silver stain , chemistry , microbiology and biotechnology , mass spectrometry , biology , computational biology , biochemistry , chromatography , gene , computer science
We have established the first public database of human primary T helper cell proteome using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. For the database, CD4+ human T cells were activated with anti-CD3+anti-CD28 antibodies and metabolically labeled with [35S]methionine for 24 h. Cells were lysed and proteins were separated by 2-DE. About 1500 protein spots were detected in the resulting 2-DE gels with silver staining, and 2000 spots with autoradiography. We have identified 91 proteins from the 2-DE gels using peptide mass fingerprinting, and annotated them to our database. The identified proteins are also linked to SWISS-PROTand NCBI protein databases. Our database is available via the Internet at http://www3.btk.utu.fi:8080/Genomics/Proteomics/Database.