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Comparative studies of various run buffers for chiral capillary electrophoresis using chiral crown ether as a chiral selector
Author(s) -
Jang Jaeho,
Cho Seung Il,
Chung Doo Soo
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200112)22:20<4362::aid-elps4362>3.0.co;2-8
Subject(s) - hydroxymethyl , tris , capillary electrophoresis , triethanolamine , chemistry , electrophoresis , chromatography , enantiomer , cationic polymerization , crown ether , amine gas treating , ether , analytical chemistry (journal) , organic chemistry , ion , biochemistry
In the capillary electrophoretic separation of primary amine enantiomers using (+)‐(18‐crown‐6)‐tetracarboxylic acid (18C6H 4 ) as a chiral selector, the presence of run buffer constituents such as tris(hydroxymethyl)aminomethane (Tris) or Na + competing with analytes for 18C6H 4 , diminishes the effectiveness of 18C6H 4 . In order to determine appropriate buffer systems for 18C6H 4 , various run buffer cationic components including Tris, 1,3‐bis[tris(hydroxymethyl)methylamino]propane, bis(2‐hydroxyethyl)iminotris(hydroxymethyl)methane, triethanolamine, tetramethylammonium, and Na + were compared. Quantitative studies of the effects of the competitive constituents were carried out by measuring the electrophoretic mobilities of histidine as a function of the 18C6H 4 concentration. We also derived a simple equation to estimate the optimal chiral selector concentration for a maximum mobility difference in the presence of a competitive inhibitor.