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Electrophoretic behavior of a highly water‐soluble dendro[60]fullerene
Author(s) -
TamisierKarolak SaraL.,
Pagliarusco Sabrina,
Herrenknecht Christine,
Brettreich Michael,
Hirsch Andreas,
Céolin René,
Bensasson René V.,
Szwarc Henri,
Moussa Fathi
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200112)22:20<4341::aid-elps4341>3.0.co;2-i
Subject(s) - capillary electrophoresis , fullerene , ionic strength , electrolyte , electrophoresis , chromatography , chemistry , taguchi methods , capillary action , chemical engineering , analytical chemistry (journal) , materials science , composite material , organic chemistry , aqueous solution , electrode , engineering
The aim of the present study was to develop an analytical method for measuring amounts of a dendro[60]fullerene (DF) which is a highly water‐soluble [60]fullerene derivative. We tried to define a straightforward methodology using capillary zone electrophoresis, a method which, to our knowledge, has not yet been used to that purpose. Preliminary assays showed that DF has almost the same mobility than the electroosmotic flow (EOF) but in the opposite direction. Attempts were carried out to reduce the EOF and positive results were obtained by adding hydroxypropylcellulose to the background electrolyte. In order to define optimal operating conditions, a Taguchi experimental plan was used to study simultaneously the effects of the main parameters that are pH, ionic strength, methanol amount and hydroxypropylcellulose concentration. Two parameters are of the utmost importance as to their effect on the migration time and separation efficiency: pH and ionic strength whose actions are opposite.