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Novel cross‐linked polyacrylamide matrices: An investigation using gradient gel electrophoresis
Author(s) -
Caulfield Marcus J.,
Purss Helen K.,
Solomon David H.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200112)22:20<4297::aid-elps4297>3.0.co;2-f
Subject(s) - monomer , polyacrylamide , polyacrylamide gel electrophoresis , chemistry , gel electrophoresis , density gradient , electrophoresis , differential (mechanical device) , chromatography , polymer chemistry , polymer , biochemistry , organic chemistry , physics , thermodynamics , enzyme , quantum mechanics
Gradient gel electrophoresis was used to examine the separation properties of novel cross‐linking compounds for polyacrylamide (PAAm). At low %T and at the same %C protein migration difference is accentuated for bismethacrylamidc cross‐linked networks relative to bisacrylamide cross‐linked networks. Similar properties were observed for cyclic monomers at low %T. This trend is maintained throughout the gradient. However, at higher %T migration differential relative to N , N ′‐methylenebisacrylamide (Bis) was less pronounced. Evidence from gradient gels suggests that reactivity and functionality of vinyl groups impose an overriding control over network formation.