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High‐throughput genotyping of repeat polymorphism in the regulatory region of serotonin transporter gene by gel microchip electrophoresis
Author(s) -
Nemoda Zsofia,
Ronai Zsolt,
Szekely Anna,
Kovacs Erika,
Shandrick Sarah,
Guttman András,
SasvariSzekely Maria
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200110)22:18<4008::aid-elps4008>3.0.co;2-#
Subject(s) - genotyping , serotonin transporter , genetics , polymorphism (computer science) , gene , biology , gel electrophoresis , microbiology and biotechnology , genotype
Large‐scale genotyping of the repeat polymorphism in the regulatory region of the serotonin transporter gene (5‐HTTLPR) was attempted by polymerase chain reaction (PCR) amplification followed by gel microchip electrophoresis analysis. The multilane (96) format of the gel microchip system allowed parallel separation of a large number of samples. The separation and visualization of the PCR amplicons from either the 5‐HTTLPR short allele (number of repeats are 14) or the 5‐HTTLPR long form (16 repeats) was completed in a few minutes. Genotyping of healthy Caucasian individuals showed that the short allele had a somewhat lower frequency (0.42) than the long form (0.58), and the genotype frequencies fulfilled the criteria of the Hardy‐Weinberg equilibrium ( = 0.012, p = 0.994). Based on these results, gel microchip electrophoresis system proved to be a powerful tool for high throughput genotyping of repeat polymorphism.