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Multiplexed enzyme assays in capillary electrophoretic single‐use microfluidic devices
Author(s) -
Xue Qifeng,
Wainright Ann,
Gangakhedkar Surekha,
Gibbons Ian
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200110)22:18<4000::aid-elps4000>3.0.co;2-a
Subject(s) - microfluidics , enzyme , capillary electrophoresis , multiplexing , chromatography , electrophoresis , chemistry , enzyme assay , single use , capillary action , biochemistry , nanotechnology , materials science , computer science , engineering , telecommunications , process engineering , composite material
We describe a method of performing multiple enzyme assays in a single reaction vessel. The resolving power of capillary electrophoresis enables several enzyme assays to be analyzed at high speed in microfluidic arrays. Multiplexed measurement can increase throughput significantly without requiring highly dense microfluidic arrays. Enzyme assays in a multiplexed format for selected kinases in this work show essentially identical performance to assays performed individually. This establishes an approach for screening one compound against multiple enzyme targets simultaneously. Another potential application for performing multiplexed enzyme assay is to study protein‐protein (especially enzyme‐enzyme) interaction by monitoring the enzymatic activity changes.