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Miniaturized multichannel electrospray ionization emitters on poly(dimethylsiloxane) microfluidic devices
Author(s) -
Kim JinSung,
Knapp Daniel R.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200110)22:18<3993::aid-elps3993>3.0.co;2-x
Subject(s) - microfluidics , electrospray , electrospray ionization , materials science , nanotechnology , chromatography , mass spectrometry , analytical chemistry (journal) , optoelectronics , chemistry
A multichannel electrospray ionization (ESI) emitter was fabricated as part of a poly(dimethylsiloxane) (PDMS) microfluidic device using a three‐layer photoresist process which also produces a self‐alignment system to make a bonding between the top and bottom PDMS parts. The prototype device (2 cm high×5 cm wide×5 cm long) had 16‐channels (30 νm wide×50 νm deep) with emitters of 1 mm length and 60° point angle. The PDMS emitter tips enabled interfacing the device to ESI‐mass spectrometry; a stable electrospray from the tips was performed with limits of detection under 1 ν M for reference peptides (adrenocorticotropic hormone fragment 1–17, angiotensin I and III).