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Disposable polymeric high‐density nanovial arrays for matrix assisted laser desorption/ionization‐ time of flight‐mass spectrometry: I. Microstructure development and manufacturing
Author(s) -
MarkoVarga György,
Ekström Simon,
Helldin Göran,
Nilsson Johan,
Laurell Thomas
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200110)22:18<3978::aid-elps3978>3.0.co;2-7
Subject(s) - mass spectrometry , materials science , microfabrication , embossing , matrix assisted laser desorption/ionization , time of flight mass spectrometry , desorption , analytical chemistry (journal) , polycarbonate , fabrication , ionization , chromatography , chemistry , composite material , medicine , ion , alternative medicine , organic chemistry , pathology , adsorption
In order to meet the expected enormous demand for mass spectrometry (MS) throughput as a result of the current efforts to completely map the human proteome, this paper presents a new concept for low‐cost high‐throughput protein identification by matrix assisted laser desorption/ionization‐time of flight‐(MALDI‐TOF)‐MS peptide mapping using disposable polymeric high‐density nanovial MALDI target plates. By means of microfabrication technology precision engineered nanovial arrays are fabricated in polymer substrates such as polymethylmethacrylate (PMMA) and polycarbonate (PC). The target plate fabrication processes investigated were precision micromilling, cold embossing and injection moulding (work in progress). Nanovial dimensions were 300, 400 or 500 νm. Typical array densities were 165 nanovials/cm 2 , which corresponds to 3300 vials on a full Applied Biosystems MALDI target plate. Obtained MALDI data displayed equal mass resolution, accuracy, signal intensity for peptide standards as compared to high‐density silicon nanovial arrays previously reported by our group [7], as well as conventional stainless steel or gold targets.